Using the FluidFM BOT system to deliver your CRISPR-Cas complex, you can selectively choose the cell you want to inject within a cell culture. Deliver your CRISPR-Cas complex directly to where it is required: the nucleus. This is performed without affecting the neighbouring cells.
Transfection of plasmids with FluidFM nanoinjection enables the delivery of genetic material even into difficult to transfect cells. In contrast to conventional transfection methods, no toxic compounds are necessary to allow the DNA vector to enter the cell. Observe the expression of your plasmid as early as 3h post injection.The FluidFM BOT system is an unmatched tool to introduce a vast variety of materials of your choice into a cell. From small molecules to proteins, RNAs, and plasmids, the FluidFM nanosyringe can be loaded with – and thus deliver – virtually any kind of liquid-based solution in a cell-context preserving, non-destructive and measurable manner.
The efficiency of the FluidFM nanoinjection has already been demonstrated in various kind of cell types such as HeLa, HEK, CHO or human iPS cells.FluidFM nanoinjection combined with other FluidFM applications, such as extraction and manipulation, makes the FluidFM BOT an incredible tool for biologists. The system has been designed specifically to address bottlenecks in the pipelines of pharmaceutical drug development and genetic editing at the single cell level.FluidFM technology unites the best features of microfluidics and force microscopy by introducing a microscopic channel into force sensitive probes.
The result is the FluidFM probe, such as the FluidFM nanosyringe, the heart of our patented FluidFM technology.Cytosurge AG successfully develops, manufactures and distributes cutting-edge and unique high-precision nanotechnology instruments as well as robotic systems based on its patented FluidFM® technology. Whether via the FluidFM ADD-ON, a powerful upgrade solution for your AFM, the FluidFM BOT, a fully integrated system for cell research, or via the award-winning FluidFM µ3Dprinter for 3D metal printing at the micrometre scale, Cytosurge provides leading-edge tools and processes to those who need or want to go beyond current technological boundaries.FluidFM – GO BEYOND.